![]() ![]() control groups, as evidenced by a neural structure spanning the 13 mm gap. The use of the delivery system (DS) with either GDNF or NGF resulted in a higher frequency of nerve regeneration vs. Nerves were harvested 6 weeks after treatment for analysis by histomorphometry and electron microscopy. Seven experimental groups were evaluated which received GDNF or nerve growth factor (NGF) with the delivery system within the conduit, control groups excluding one or more components of the delivery system, and nerve isografts. In this study we evaluated the effect of affinity-based delivery of GDNF from a fibrin matrix in a nerve guidance conduit on nerve regeneration in a 13 mm rat sciatic nerve defect. The delivery of GDNF to the peripheral nervous system has been shown to enhance regeneration following injury. Glial-derived neurotrophic factor (GDNF) promotes both sensory and motor neuron survival. The neurohistopathological results that showed the number of Schwann cells proliferation and orientation of regenerative nerve fibers were observed most clear and improved in Cytidine monophosphate. ![]() The conductive velocity on 16 th weeks postoperative, the results showed significant differences in the same group between right sciatic nerve (operated) and left sciatic nerve and significant differences between groups at (p<0.05) were the best conductivity in Cytidine monophosphate group compared with other groups. Moreover, the electrophysiological studies were carried out by isolation of the sciatic nerve. The clinical assessment of motor and sensory nerve functions revealed significant differences between the Cytidine monophosphate and control group, the Cytidine monophosphate more effective than Platelet rich plasma group. The histopathological examination of regenerated nerve sections (three parts of 1 cm long each) were collected from the proximal, middle (injured site) and distal segments and used to determine the degree of the sciatic nerve regeneration. The conductive velocity was measured at the end of study and macroscopic evaluation demonstrated a degree of nerve stump coaptation, adhesion, thickness and the presence of neuroma. The clinical examination (motor and sensory function) showed occurrence of onset, gait, and knuckling that were evaluated weekly from the starting until the end of the study (sixteen week). The sciatic nerve axotomy was done on the right hind limb and then the nerve sutured immediately by nylon suture 0-5 with end-to-end anastomosis, using simple interrupted suture, and then the Cytidine monophosphate group the dogs were injected with Cytidine monophosphate 5 mg/day intramuscular intake for 30 day post operation. They were randomly divided into two equal groups, control group and Cytidine Monophosphate group. Eight healthy adult dogs were used to complete this study. The objective of the present research was to evaluate the efficacy of Cytidine Monophosphate on the regeneration of sciatic nerve injury of dogs. ![]()
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